Dengue is one of the most widespread mosquito-borne infections in Malaysia. The diagnosis still remains a challenge in area of disease confirmation where it could not be easily differentiated with other febrile illnesses even though the diagnosis of classical dengue fever and dengue haemorrhagic fever can be recognized clinically.
The clinical diagnosis also can be difficult where the signs and symptoms presented are easily confused with malaria, leptospirosis and typhoid fever. Therefore, an early and effective evaluation of the peripheral blood can be very helpful in patient management.
The objectives of this study are to determine the morphological features in peripheral blood film (PBF) of pathogenic dengue infection. 30 PBF of positive dengue infection in University Malaya Medical centre (UMMC) had been examined in this study where atypical lymphocytes [n=27, (90%)] and thrombocytopenia [n=22, (73.3%)] were consistently found. Presence of thrombocytopenia and presence of atypical lymphocytes in PBF are important diagnostic clues for early diagnosis of dengue infection which could be potentially useful parameter in screening dengue. Therefore, PBF can have a signi?cant function in supporting the diagnosis of dengue which can act as complement to the full blood count and serological diagnosis of dengue especially in cases where the clinical manifestation are abnormal.
Azeredo, E. L., Monteiro, R. Q., & Pinto, L. M. (2009). Thrombocytopenia in dengue: Interrelationship between virus and the imbalance between coagulation and fibrinolysis and inflammatory mediators. Hematology and Fundamentals of Hemostasis, 1(2), 93–116.
Curry, C., & Staros, E. (2012). Mean corpuscular volume (MCV). Retrieved from http://emedicine.medscape.com/
Hunsperger, E. A., Yoksan, S., Buchy, P., Nguyen, V. C., Sekaran, S. D., & Enria, D. A. (2009). Evaluation of commercially available anti-dengue virus immunoglobulin. Emerg Infect Dis, 15(3), 436–40.
Jampangern, W., Vongthoung, K., Jittmittraphap, A., Worapongpaiboon, S., Limkittikul, K., Chuansumrit, A., Tarunotai, U., & Chongsa-nguan, M. (2007). Characterization of atypical lymphocytes and immunophenotypes of lymphocytes in patients with dengue virus infection. Asian Pacific J Allergy Immunol, 25(1), 27–36.
Jones, K. W. (2006). Evaluation of cell morphology and introduction to platelet and white blood cell morphology. Blood J, 98(4), 1273–1275.
Mehta, R. C., Goswami, H. M., Katara, R. K., Patel, P. S., Parikh, U. V., & Vegad, M. M. (2013). Peripheral smear examination in early diagnosis of dengue patients. Adv Virol, 2(1), 22–24.
Noisakran, S., Onlamoon, N., Songprakhon, P., Hsiao, H. M., Chokephaibulkit, K., & Perng, G. C. (2010). Cells in dengue virus infection in vivo. Adv Virol. doi: 10.1155/2010/164878
Smith, A. W., & Schwartz, E. (2005). Dengue in travelers. N Engl J Med, 353, 924–932.
Yunus, Y. M., Narowi, M., & Lai, H. I. (2017). Morphological Features Analysis in Pathogenic Dengue Infection as an Alternative Screening Method. International Journal of Academic Research in Business and Social Sciences, 7(2), 706-715.
Copyright: © 2017 The Author(s)
Published by Human Resource Management Academic Research Society (www.hrmars.com)
This article is published under the Creative Commons Attribution (CC BY 4.0) license. Anyone may reproduce, distribute, translate and create derivative works of this article (for both commercial and non-commercial purposes), subject to full attribution to the original publication and authors. The full terms of this license may be seen at: http://creativecommons.org/licences/by/4.0/legalcode
