New human immunodeficiency virus (HIV) infection and HIV prevalence is still a problem in Nyanza Region, Kenya. Part of the reason for this is failure of Non-governmental organizations (NGOs) to craft robust organizational purpose that can drive positive outcomes. The purpose of the study was to examine the influence of organization’s purpose on performance of HIV and Acquired Immune-Deficiency Syndrome (AIDS) interventions managed by NGOs in Nyanza Region and the moderating influence of stakeholder participation on such relationship. The study adopted ex-post facto survey design. The study population encompassed 60 departmental heads at NGO level as well as six directors from three NGO national coordinating bodies in Kenya. The study applied purposive sampling techniques to pick the respondents for the study. The data collection instruments for the study comprised: self-administered questionnaires and Key informant interview guides. It was found out that there was a significant weak positive relationship between organization’s purpose and the performance of HIV and AIDS interventions, r (50) =0.094, p= 0.008,CL=95%. Stakeholder participation significantly moderated the relationship between organization’s purpose and performance of HIV and AIDS interventions managed by NGOs in Nyanza Region, critical value was= 3.84 and the X2(1) value =5.030, 3.84 < 5.030.The study concluded crafting organization’s purpose in most organisation’s was not effective. Stakeholder participation was also not optimal. It is recommended that NGOs implementing HIV and AIDS interventions need to relook into the process of coming up with the vision, mission and core values and stakeholder participation in crafting organisation’s purpose.
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