One of the major challenges in biology education is students’ difficulty in understanding the abstract concepts of cell division. Many struggles with the sequential stages of mitosis and meiosis, as well as the molecular basis of processes such as crossing over. Traditional lecture methods are often ineffective in addressing these complexities, resulting in persistent misconceptions. Furthermore, standardized assessments typically emphasize lower-order questions, which do not adequately capture students’ conceptual understanding of cell division. Therefore, this study aimed to develop and evaluate the Cell Division Escape kit, a game-based learning aid for Form Four Biology, with a focus on its validity and usability. A developmental research design guided by the ADDIE instructional model was employed. The instruments used were an expert validation form and a usability questionnaire. Two Biology experts, one from Universiti Pendidikan Sultan Idris and another from Pahang Matriculation College, evaluated the kit for content validity. The usability of the kit was tested with 32 Form Four Biology students, while a pilot study involving 12 students was conducted to establish reliability. Data were analyzed using the Content Validity Index (CVI), Cronbach’s alpha, and descriptive statistics via SPSS Version 29.0. The results showed that the Cell Division Escape kit achieved a CVI value of 1.00, an overall Cronbach’s alpha of 0.97, and a high usability level with a mean score of 3.78. These findings demonstrate that the kit possesses high validity, strong reliability, and satisfactory usability. In conclusion, the kit provides a practical and engaging teaching tool to enhance students’ understanding of cell division.
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